Titre :	Développement et valorisation des activités biologiques d'une formulation à base de l'huile essentielle d'arbre à thé 'Malaleuca alternifolia)
Type de document :	texte imprimé
Auteurs :	Safaa Ben Hadj Taher, Auteur ; Achouak Hour El Aine Hasnaoui, Auteur ; Guici EL kouacheur khadidja, Auteur
Editeur :	Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-
Année de publication :	2024/2025
Importance :	p.101
Présentation :	couv. en noir et blanc ., ill., fig.,tabl.
Format :	30 cm
Accompagnement :	CD
Note générale :	Biliographie: p71-88. Annexes: p89-99.
Langues :	Français (fre)
Catégories :	Biochimie
Mots-clés :	Melaleuca alternifolia  nanoemulsion  antioxidant  anti-inflammatory  anti- infective, wound infection  S.aureus
Index. décimale :	572.12
Résumé :	This study aims to evaluate the essential oil (EO) of Melaleuca alternifolia (tea tree) through the development of an innovative nanoformulation by assessing its antioxidant, anti-inflammatory and anti-infectious activities with a view to cosmetic and therapeutic applications. GC-MS chromatographic analysis identified seven constituents, including terpinen- 4-ol (34.01%) as the majority compound, followed by γ-terpinene (20.60%) and α- terpinene (9.99%). Assessment of in vitro antioxidant activity, using the DPPH method, revealed low to moderate antioxidant power. The anti-inflammatory effect was examined using a mouse model of xylene-induced auricular edema. The results showed significant inhibition of inflammation, with reduction rates of 70.30% and 60.63% for nanoemulsions containing 1% and 0.5% EO respectively. These results were corroborated by histological observations, demonstrating a significant reduction in inflammatory infiltrates in treated tissues. At the same time, anti-infectious activity was assessed in vivo on a model of cutaneous wounds infected with Staphylococcus aureus. The cream formulated with 1% EO promoted accelerated wound healing, with a wound surface reduction rate of 96.06%, surpassing those observed with Fucidine® (90.17%) and the 0.5% formulation (88.70%), illustrating a dose-dependent effect. Histological examination on day 9 confirmed more advanced re-epithelialization and a significant reduction in bacterial load in tissues treated with the highest concentration. In addition, the aromatogram showed significant antibacterial activity of pure EO, with an inhibition zone diameter of 28.5 mm against S.aureus. In conclusion, these results underline the therapeutic potential of M. alternifolia EO-based nanoemulsion as a promising natural alternative in the treatment of skin inflammations and bacterial infections, particularly those caused by S.aureus.

Développement et valorisation des activités biologiques d'une formulation à base de l'huile essentielle d'arbre à thé 'Malaleuca alternifolia) [texte imprimé] / Safaa Ben Hadj Taher, Auteur ; Achouak Hour El Aine Hasnaoui, Auteur ; Guici EL kouacheur khadidja, Auteur . - Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-, 2024/2025 . - p.101 : couv. en noir et blanc ., ill., fig.,tabl. ; 30 cm + CD.
Biliographie: p71-88.
Annexes: p89-99.
Langues : Français (fre)

Catégories :	Biochimie
Mots-clés :	Melaleuca alternifolia  nanoemulsion  antioxidant  anti-inflammatory  anti- infective, wound infection  S.aureus
Index. décimale :	572.12
Résumé :	This study aims to evaluate the essential oil (EO) of Melaleuca alternifolia (tea tree) through the development of an innovative nanoformulation by assessing its antioxidant, anti-inflammatory and anti-infectious activities with a view to cosmetic and therapeutic applications. GC-MS chromatographic analysis identified seven constituents, including terpinen- 4-ol (34.01%) as the majority compound, followed by γ-terpinene (20.60%) and α- terpinene (9.99%). Assessment of in vitro antioxidant activity, using the DPPH method, revealed low to moderate antioxidant power. The anti-inflammatory effect was examined using a mouse model of xylene-induced auricular edema. The results showed significant inhibition of inflammation, with reduction rates of 70.30% and 60.63% for nanoemulsions containing 1% and 0.5% EO respectively. These results were corroborated by histological observations, demonstrating a significant reduction in inflammatory infiltrates in treated tissues. At the same time, anti-infectious activity was assessed in vivo on a model of cutaneous wounds infected with Staphylococcus aureus. The cream formulated with 1% EO promoted accelerated wound healing, with a wound surface reduction rate of 96.06%, surpassing those observed with Fucidine® (90.17%) and the 0.5% formulation (88.70%), illustrating a dose-dependent effect. Histological examination on day 9 confirmed more advanced re-epithelialization and a significant reduction in bacterial load in tissues treated with the highest concentration. In addition, the aromatogram showed significant antibacterial activity of pure EO, with an inhibition zone diameter of 28.5 mm against S.aureus. In conclusion, these results underline the therapeutic potential of M. alternifolia EO-based nanoemulsion as a promising natural alternative in the treatment of skin inflammations and bacterial infections, particularly those caused by S.aureus.