Titre :	Caractérisation biochimique et évaluation de quelques activités biologiques de l'huile essentielle de Ciste Ladanifère (Cistus ladanifer)
Type de document :	texte imprimé
Auteurs :	Yasmine Fouzia Boukhenoufa, Auteur ; Abir Boufrioua, Auteur ; Guici EL kouacheur khadidja, Auteur
Editeur :	Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-
Année de publication :	2024/2025
Importance :	p.85
Présentation :	couv. en noir et blanc ., ill., fig.,tabl.
Format :	30 cm
Accompagnement :	CD
Note générale :	Bibliographie: p65-78. Annexes: p79-83.
Langues :	Français (fre)
Catégories :	Biochimie
Mots-clés :	Cistus ladanifer  Nano-emulsion  GC/MS  Biological activities  Irritation test.
Index. décimale :	572.07
Résumé :	This final year project focused on the analysis of Cistus ladanifer essential oil, highlighting its chemical composition, physicochemical properties, and some biological activities, with the aim of enhancing its therapeutic potential through innovative formulations. The analysis by GC-MS allowed the identification of the major compounds present in the essential oil of C. ladanifer. The results revealed a predominance of monoterpenes. The pH of the two nano-emulsions, close to 6, indicates good compatibility with the skin. The measured zeta potential confirms their stability and nanometric nature. On the biological level, several tests were conducted to determine the pharmacological properties of the formulations. The antioxidant activity was evaluated using the DPPH method, revealing a moderate capacity for trapping free radicals, indicating a low antioxidant activity. On the other hand, the anti-inflammatory effect yielded promising results, tested using a mouse ear edema model induced by xylene, it showed a significant inhibition of edema, reaching 69.41% with 1.5% NE, its strong anti-inflammatory potential was subsequently confirmed by histological analyses. The wound healing activity was studied using an in vivo skin wound model, treated topically for 14 days. The results revealed rapid and effective healing, particularly with the 1.5% NE, which promoted notable skin repair with a percentage of 88.81%. Histological analyses further confirmed tissue regeneration, showing complete and healthy re-epithelialization of the epidermis. To evaluate the tolerance and safety of NEs, two tests were conducted. The HET-CAM test revealed no ocular irritation, while the Draize test showed that the 0.5% formulation was non-irritating and the 1.5% formulation was slightly irritating, highlighting a safe and secure use. This study highlights that the essential oil of C. ladanifer possesses interesting biological properties, particularly anti-inflammatory and healing, while being generally well tolerated on the skin. Although its antioxidant activity is low, the physicochemical stability of the formulations and their potential therapeutic efficacy confirm the medicinal value of this plant, paving the way for its integration into innovative topical products for dermatological use.

Caractérisation biochimique et évaluation de quelques activités biologiques de l'huile essentielle de Ciste Ladanifère (Cistus ladanifer) [texte imprimé] / Yasmine Fouzia Boukhenoufa, Auteur ; Abir Boufrioua, Auteur ; Guici EL kouacheur khadidja, Auteur . - Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-, 2024/2025 . - p.85 : couv. en noir et blanc ., ill., fig.,tabl. ; 30 cm + CD.
Bibliographie: p65-78.
Annexes: p79-83.
Langues : Français (fre)

Catégories :	Biochimie
Mots-clés :	Cistus ladanifer  Nano-emulsion  GC/MS  Biological activities  Irritation test.
Index. décimale :	572.07
Résumé :	This final year project focused on the analysis of Cistus ladanifer essential oil, highlighting its chemical composition, physicochemical properties, and some biological activities, with the aim of enhancing its therapeutic potential through innovative formulations. The analysis by GC-MS allowed the identification of the major compounds present in the essential oil of C. ladanifer. The results revealed a predominance of monoterpenes. The pH of the two nano-emulsions, close to 6, indicates good compatibility with the skin. The measured zeta potential confirms their stability and nanometric nature. On the biological level, several tests were conducted to determine the pharmacological properties of the formulations. The antioxidant activity was evaluated using the DPPH method, revealing a moderate capacity for trapping free radicals, indicating a low antioxidant activity. On the other hand, the anti-inflammatory effect yielded promising results, tested using a mouse ear edema model induced by xylene, it showed a significant inhibition of edema, reaching 69.41% with 1.5% NE, its strong anti-inflammatory potential was subsequently confirmed by histological analyses. The wound healing activity was studied using an in vivo skin wound model, treated topically for 14 days. The results revealed rapid and effective healing, particularly with the 1.5% NE, which promoted notable skin repair with a percentage of 88.81%. Histological analyses further confirmed tissue regeneration, showing complete and healthy re-epithelialization of the epidermis. To evaluate the tolerance and safety of NEs, two tests were conducted. The HET-CAM test revealed no ocular irritation, while the Draize test showed that the 0.5% formulation was non-irritating and the 1.5% formulation was slightly irritating, highlighting a safe and secure use. This study highlights that the essential oil of C. ladanifer possesses interesting biological properties, particularly anti-inflammatory and healing, while being generally well tolerated on the skin. Although its antioxidant activity is low, the physicochemical stability of the formulations and their potential therapeutic efficacy confirm the medicinal value of this plant, paving the way for its integration into innovative topical products for dermatological use.