Titre :	Biosynthesis, in vitro, in silico and in vivo study of the antifungal activity of some nanomaterials against the potato dry rot causal agent Fusarium sp
Type de document :	texte imprimé
Auteurs :	Sarah Bouchireb, Auteur ; Faiza Meslek, Auteur ; Amel Midoune, Auteur
Editeur :	Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-
Année de publication :	2024/2025
Importance :	p.72
Présentation :	couv. en noir et blanc ., ill., fig.,tabl.
Format :	30 cm
Accompagnement :	CD
Note générale :	Bibliographie: p53-63. Annexes: p64-72.
Langues :	Français (fre)
Catégories :	Agronomie
Mots-clés :	nanomateials  antifungal activity  Fusarium sp  in silico  in vitro  in vivo.
Index. décimale :	630.13
Résumé :	This study explores the fungi-mediated biosynthesis of copper oxide (CuO) nanoparticles and the formulation of a nanoemulsion based on Pelargonium graveolens essential oil, with a focus on evaluating their antifungal activity. CuO nanoparticles were synthesized using culture filtrates of Trichoderma sp. isolate Tr5, while the essential oil nanoemulsion was prepared via ultrasonication. The antifungal efficacy of these nanomaterials was assessed in vitro following the poisoned food technique against a Fusarium sp. isolate, and their effects on mycelial morphology were examined microscopically. The essential oil nanoemulsion demonstrated remarkable inhibitory activity, achieving approximately 100% inhibition of mycelial growth across all tested concentrations (0.0625%, 0.125 %, 0.25 %, 0.5% and 1%), whereas CuONPS exhibited only moderate inhibition at high concentrations recording 35 % and and 20% of inhibition at 160µg/ ml and 320 µg/ ml respectively. Carbendazim also achieved complete inhibition at all concentrations, while copper oxychloride matched this efficacy only at the highest concentration tested. Microscopic analysis revealed significant cellular deformation and disorganization in mycelium treated with effective doses of the nanomaterials. Molecular docking studies indicated notable interactions between β-Citronellol, the principal component of the essential oil, and Fusarium target proteins, particularly Chitin synthetase (glide score of -5.655). In vivo experiments using Spunta potato tuber slices corroborated the in vitro findings, as both the EO nanoemulsion and Carbendazim effectively prevented lesion development. These results highlight the potential of Pelargonium graveolens EOnanoemulsion as a promising antifungal agent.

Biosynthesis, in vitro, in silico and in vivo study of the antifungal activity of some nanomaterials against the potato dry rot causal agent Fusarium sp [texte imprimé] / Sarah Bouchireb, Auteur ; Faiza Meslek, Auteur ; Amel Midoune, Auteur . - Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-, 2024/2025 . - p.72 : couv. en noir et blanc ., ill., fig.,tabl. ; 30 cm + CD.
Bibliographie: p53-63.
Annexes: p64-72.
Langues : Français (fre)

Catégories :	Agronomie
Mots-clés :	nanomateials  antifungal activity  Fusarium sp  in silico  in vitro  in vivo.
Index. décimale :	630.13
Résumé :	This study explores the fungi-mediated biosynthesis of copper oxide (CuO) nanoparticles and the formulation of a nanoemulsion based on Pelargonium graveolens essential oil, with a focus on evaluating their antifungal activity. CuO nanoparticles were synthesized using culture filtrates of Trichoderma sp. isolate Tr5, while the essential oil nanoemulsion was prepared via ultrasonication. The antifungal efficacy of these nanomaterials was assessed in vitro following the poisoned food technique against a Fusarium sp. isolate, and their effects on mycelial morphology were examined microscopically. The essential oil nanoemulsion demonstrated remarkable inhibitory activity, achieving approximately 100% inhibition of mycelial growth across all tested concentrations (0.0625%, 0.125 %, 0.25 %, 0.5% and 1%), whereas CuONPS exhibited only moderate inhibition at high concentrations recording 35 % and and 20% of inhibition at 160µg/ ml and 320 µg/ ml respectively. Carbendazim also achieved complete inhibition at all concentrations, while copper oxychloride matched this efficacy only at the highest concentration tested. Microscopic analysis revealed significant cellular deformation and disorganization in mycelium treated with effective doses of the nanomaterials. Molecular docking studies indicated notable interactions between β-Citronellol, the principal component of the essential oil, and Fusarium target proteins, particularly Chitin synthetase (glide score of -5.655). In vivo experiments using Spunta potato tuber slices corroborated the in vitro findings, as both the EO nanoemulsion and Carbendazim effectively prevented lesion development. These results highlight the potential of Pelargonium graveolens EOnanoemulsion as a promising antifungal agent.