| Titre : |
Activitéantagoniste de l'extrait biologique de serratia marcescens vis-à-vis les germes multésistants et ses activités biologiques |
| Type de document : |
texte imprimé |
| Auteurs : |
Sabrine Hathout, Auteur ; aicha Debib, Auteur |
| Editeur : |
Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza- |
| Année de publication : |
2024/2025 |
| Importance : |
p.80 |
| Présentation : |
couv. en noir et blanc ., ill., fig. |
| Format : |
30 cm |
| Accompagnement : |
CD |
| Note générale : |
Bibliographie: p66-74.
Annexes: p75-80. |
| Langues : |
Français (fre) |
| Catégories : |
Microbiologie
|
| Mots-clés : |
Serratia marcescens prodigiosin ZnNPs multidrug resistance therapeutic potential. |
| Index. décimale : |
579.47 |
| Résumé : |
As part of the fight against multidrug-resistant infections and the development of biologically derived therapeutic agents, this study focused on the bioactive properties of a pigment extract from Serratia marcescens and its biosynthesized zinc nanoparticles (ZnNPs). The main objective was to evaluate their efficacy across various biological activities; antimicrobial, antibiofilm, antifungal, antioxidant, anti-inflammatory, anti-hemolytic, and insecticidal activity.
The extract, rich in prodigiosin, showed marked antimicrobial activity against several multidrug-resistant strains. Agar diffusion revealed a 23 mm inhibition zone against methicillin- resistant Staphylococcus aureus (MRSA). MIC values confirmed this efficacy, ranging from 0.15 mg/mL (Klebsiella pneumoniae) to 1.25 mg/mL (Acinetobacter baumannii). ZnNPs showed enhanced efficacy against some strains, reducing the MIC of A. baumannii from 1.25 to 0.62 mg/mL.
In antibiofilm assays, over 50% inhibition was observed for A. baumannii at 5 mg/mL of extract, with comparable effects from ZnNPs at 2.5 mg/mL. Antifungal activity showed that ZnNPs were significantly effective against Aspergillus niger and A. carbonarius (MIC: 0.026 mg/mL), while Candida albicans appeared more resistant (MIC: 0.62 mg/mL).
Antioxidant activity assessed by the DPPH method yielded IC50 values of 3.89 mg/mL for the extract and 4.6 mg/mL for ZnNPs, outperforming some earlier studies. For anti-inflammatory activity, ZnNPs inhibited up to 97% of egg albumin denaturation, exceeding diclofenac (85%). Anti- hemolytic activity revealed strong thermal protection at 60°C by ZnNPs (~75%) compared to <20% for the crude extract.
Lastly, the insecticidal activity against Tribolium castaneum showed over 85% mortality with ZnNPs at 2.5 mg/mL after 72 h, and about 70% with the extract at 0.5 mg/mL. Two-way ANOVA confirmed highly significant effects (p < 0.0001) of both concentration and exposure time on mortality, with concentration contributing 81.11%.
These results highlight the high therapeutic potential of S. marcescens and its ZnNPs, which deserve further attention as alternative antimicrobial and biocontrol agents.
|
Activitéantagoniste de l'extrait biologique de serratia marcescens vis-à-vis les germes multésistants et ses activités biologiques [texte imprimé] / Sabrine Hathout, Auteur ; aicha Debib, Auteur . - Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-, 2024/2025 . - p.80 : couv. en noir et blanc ., ill., fig. ; 30 cm + CD. Bibliographie: p66-74.
Annexes: p75-80. Langues : Français ( fre)
| Catégories : |
Microbiologie
|
| Mots-clés : |
Serratia marcescens prodigiosin ZnNPs multidrug resistance therapeutic potential. |
| Index. décimale : |
579.47 |
| Résumé : |
As part of the fight against multidrug-resistant infections and the development of biologically derived therapeutic agents, this study focused on the bioactive properties of a pigment extract from Serratia marcescens and its biosynthesized zinc nanoparticles (ZnNPs). The main objective was to evaluate their efficacy across various biological activities; antimicrobial, antibiofilm, antifungal, antioxidant, anti-inflammatory, anti-hemolytic, and insecticidal activity.
The extract, rich in prodigiosin, showed marked antimicrobial activity against several multidrug-resistant strains. Agar diffusion revealed a 23 mm inhibition zone against methicillin- resistant Staphylococcus aureus (MRSA). MIC values confirmed this efficacy, ranging from 0.15 mg/mL (Klebsiella pneumoniae) to 1.25 mg/mL (Acinetobacter baumannii). ZnNPs showed enhanced efficacy against some strains, reducing the MIC of A. baumannii from 1.25 to 0.62 mg/mL.
In antibiofilm assays, over 50% inhibition was observed for A. baumannii at 5 mg/mL of extract, with comparable effects from ZnNPs at 2.5 mg/mL. Antifungal activity showed that ZnNPs were significantly effective against Aspergillus niger and A. carbonarius (MIC: 0.026 mg/mL), while Candida albicans appeared more resistant (MIC: 0.62 mg/mL).
Antioxidant activity assessed by the DPPH method yielded IC50 values of 3.89 mg/mL for the extract and 4.6 mg/mL for ZnNPs, outperforming some earlier studies. For anti-inflammatory activity, ZnNPs inhibited up to 97% of egg albumin denaturation, exceeding diclofenac (85%). Anti- hemolytic activity revealed strong thermal protection at 60°C by ZnNPs (~75%) compared to <20% for the crude extract.
Lastly, the insecticidal activity against Tribolium castaneum showed over 85% mortality with ZnNPs at 2.5 mg/mL after 72 h, and about 70% with the extract at 0.5 mg/mL. Two-way ANOVA confirmed highly significant effects (p < 0.0001) of both concentration and exposure time on mortality, with concentration contributing 81.11%.
These results highlight the high therapeutic potential of S. marcescens and its ZnNPs, which deserve further attention as alternative antimicrobial and biocontrol agents.
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579.01 
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