| Titre : |
Évaluation de l'activité antibactérienne et antibiofilm d'une nano-formulation à base d'huile essentielle d'Artemisia alba |
| Type de document : |
texte imprimé |
| Auteurs : |
Nadine Lahlah, Auteur ; Zahra El Mouna Merouane, Auteur |
| Editeur : |
Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza- |
| Année de publication : |
2024/2025 |
| Importance : |
p.59 |
| Présentation : |
couv. en noir et blanc ., ill., fig.,tabl. |
| Format : |
30 cm |
| Accompagnement : |
CD |
| Note générale : |
Bibliographie: p53-59. |
| Langues : |
Français (fre) |
| Catégories : |
Microbiologie
|
| Mots-clés : |
Artemisia herba alba Gas chromatography/mass spectrometry Nanoemulsion Antibacterial activity Antibiofilm activity. |
| Index. décimale : |
579.41 |
| Résumé : |
This thesis focused on an in-depth study of the antibacterial and anti-biofilm properties of Artemisia herba-alba essential oil, as well as an evaluation of the impact of its nanoformulation on these biological activities. In a context marked by the growing emergence of microbial resistance and the difficulty of treating biofilm-associated infections, the exploration of natural and innovative solutions is of particular interest.
The essential oil was characterized by gas chromatography-mass spectrometry (GC-MS). The analysis revealed a rich chemical composition representing 77.17% of the total composition of 79 compounds, dominated by oxygenated monoterpenes (48.93%), with thujone (14.76%) and camphor (13.82%) as the main constituents. To improve the stability, solubility, and efficacy of the essential oil, an oil-in-water nanoemulsion was prepared using ultrasonication. The measured physicochemical parameters showed an average droplet size of 248.2 nm and a polydispersity index of 0.453, indicating homogeneous dispersion. The measured zeta potential was –11.2 mV, which corresponds to moderate electrostatic stability.
Antibacterial activity tests were performed using the aromatogram method, evaluating the diameters of the inhibition zones against several bacterial strains. The essential oil exhibited
diameters ranging from 9.33 mm against Pseudomonas koreensis to 24.33 mm against Enterococcus durans. The nanoformulation demonstrated increased efficacy, reaching a maximum diameter of 32.33 mm against Enterococcus durans, reflecting a significant increase in antimicrobial activity after nanoformulation.
The anti-biofilm potency study was conducted using polystyrene microplates, allowing for estimation of the inhibition of biofilm formation. The results showed that the nanoformulation exerted significantly greater inhibition than the essential oil, even at lower concentrations. For example, against Bacillus cereus, inhibition remained above 80% at a concentration of 12.5%, while the essential oil exhibited reduced efficacy at this same dilution. Similarly, for Escherichia coli and Streptococcus oralis, the nanoemulsion retained significant activity at low concentrations, confirming its potential as an anti-biofilm agent. These results highlight the remarkable potential of Artemisia herba-alba essential oil, whose efficacy is further amplified by nanoformulation. This innovative approach could contribute to the development of new therapeutic alternatives of natural origin, adapted to combat multidrug-resistant microbial infections and the persistence of biofilms on biological and inert surfaces.
|
Évaluation de l'activité antibactérienne et antibiofilm d'une nano-formulation à base d'huile essentielle d'Artemisia alba [texte imprimé] / Nadine Lahlah, Auteur ; Zahra El Mouna Merouane, Auteur . - Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-, 2024/2025 . - p.59 : couv. en noir et blanc ., ill., fig.,tabl. ; 30 cm + CD. Bibliographie: p53-59. Langues : Français ( fre)
| Catégories : |
Microbiologie
|
| Mots-clés : |
Artemisia herba alba Gas chromatography/mass spectrometry Nanoemulsion Antibacterial activity Antibiofilm activity. |
| Index. décimale : |
579.41 |
| Résumé : |
This thesis focused on an in-depth study of the antibacterial and anti-biofilm properties of Artemisia herba-alba essential oil, as well as an evaluation of the impact of its nanoformulation on these biological activities. In a context marked by the growing emergence of microbial resistance and the difficulty of treating biofilm-associated infections, the exploration of natural and innovative solutions is of particular interest.
The essential oil was characterized by gas chromatography-mass spectrometry (GC-MS). The analysis revealed a rich chemical composition representing 77.17% of the total composition of 79 compounds, dominated by oxygenated monoterpenes (48.93%), with thujone (14.76%) and camphor (13.82%) as the main constituents. To improve the stability, solubility, and efficacy of the essential oil, an oil-in-water nanoemulsion was prepared using ultrasonication. The measured physicochemical parameters showed an average droplet size of 248.2 nm and a polydispersity index of 0.453, indicating homogeneous dispersion. The measured zeta potential was –11.2 mV, which corresponds to moderate electrostatic stability.
Antibacterial activity tests were performed using the aromatogram method, evaluating the diameters of the inhibition zones against several bacterial strains. The essential oil exhibited
diameters ranging from 9.33 mm against Pseudomonas koreensis to 24.33 mm against Enterococcus durans. The nanoformulation demonstrated increased efficacy, reaching a maximum diameter of 32.33 mm against Enterococcus durans, reflecting a significant increase in antimicrobial activity after nanoformulation.
The anti-biofilm potency study was conducted using polystyrene microplates, allowing for estimation of the inhibition of biofilm formation. The results showed that the nanoformulation exerted significantly greater inhibition than the essential oil, even at lower concentrations. For example, against Bacillus cereus, inhibition remained above 80% at a concentration of 12.5%, while the essential oil exhibited reduced efficacy at this same dilution. Similarly, for Escherichia coli and Streptococcus oralis, the nanoemulsion retained significant activity at low concentrations, confirming its potential as an anti-biofilm agent. These results highlight the remarkable potential of Artemisia herba-alba essential oil, whose efficacy is further amplified by nanoformulation. This innovative approach could contribute to the development of new therapeutic alternatives of natural origin, adapted to combat multidrug-resistant microbial infections and the persistence of biofilms on biological and inert surfaces.
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579.01 
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