| Titre : |
L'évaluation de la toxicité et l'activité anti-inflammatoire d'une formulation à base d'helichrysum italicum |
| Type de document : |
texte imprimé |
| Auteurs : |
Malek Serhoudji, Auteur ; Cheimaa Bouchekouk, Auteur |
| Editeur : |
Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza- |
| Année de publication : |
2024/2025 |
| Importance : |
p.102 |
| Présentation : |
couv. en noir et blanc ., ill., fig.,tabl. |
| Format : |
30 cm |
| Accompagnement : |
CD |
| Note générale : |
Bibliographie: p82-96.
Annexes: p98-102. |
| Langues : |
Français (fre) |
| Catégories : |
Microbiologie
|
| Mots-clés : |
Helichrysum italicum Essential oil GC-MS Nanoemulsion anti-inflammatory acute toxicity. |
| Index. décimale : |
579.37 |
| Résumé : |
The use of essential oils is frequently limited by their high volatility, which compromises the stability and integrity of the bioactive compounds they contain. To overcome this constraint, nanoformulation is emerging as an innovative and effective strategy, aiming to improve the protection and controlled release of active ingredients. The objective of this work is to promote the essential oil of Helichrysum italicum, a Mediterranean species found in Algeria, as well as its nanoemulsion, by exploring their potential as an effective and non-toxic anti-inflammatory agent.
The chemical profile of H. italicum essential oil, commercially obtained and analyzed by gas chromatography-mass spectrometry (GC-MS), revealed the presence of 61 compounds representing 90.48% of the total composition. Sesquiterpenes, notably !-caryophyllene and "-caryophyllene, predominate, followed by linalyl acetate, !-pinene, and D-limonene. A nanoemulsion was developed from this essential oil using an environmentally friendly, high-energy method based on ultrasonication. Characterization of the nanoemulsion by dynamic light scattering (DLS) showed that it has nanometer-sized droplets with an average diameter of 149.4 nm and a negative surface charge of -16.8 mV, thus ensuring slightly moderate colloidal stability. In vivo anti-inflammatory activity was evaluated using the carrageenan-induced edema model in NMRI mice. Experimental results showed that after 5 hours, the two highest concentrations of the essential oil (50% and 25 %), as well as the highest concentration of the nanoemulsion (25%), exhibited superior anti-inflammatory activity compared to diclofenac, used as the standard treatment. In parallel, the histological study of tissue toxicity confirmed the absence of signs of acute toxicity after 14 days, thus attesting to the good tolerance of the essential oil under the experimental conditions.
|
L'évaluation de la toxicité et l'activité anti-inflammatoire d'une formulation à base d'helichrysum italicum [texte imprimé] / Malek Serhoudji, Auteur ; Cheimaa Bouchekouk, Auteur . - Tipaza [Algérie] : Centre universitaire Morsli Abdellah - Tipaza-, 2024/2025 . - p.102 : couv. en noir et blanc ., ill., fig.,tabl. ; 30 cm + CD. Bibliographie: p82-96.
Annexes: p98-102. Langues : Français ( fre)
| Catégories : |
Microbiologie
|
| Mots-clés : |
Helichrysum italicum Essential oil GC-MS Nanoemulsion anti-inflammatory acute toxicity. |
| Index. décimale : |
579.37 |
| Résumé : |
The use of essential oils is frequently limited by their high volatility, which compromises the stability and integrity of the bioactive compounds they contain. To overcome this constraint, nanoformulation is emerging as an innovative and effective strategy, aiming to improve the protection and controlled release of active ingredients. The objective of this work is to promote the essential oil of Helichrysum italicum, a Mediterranean species found in Algeria, as well as its nanoemulsion, by exploring their potential as an effective and non-toxic anti-inflammatory agent.
The chemical profile of H. italicum essential oil, commercially obtained and analyzed by gas chromatography-mass spectrometry (GC-MS), revealed the presence of 61 compounds representing 90.48% of the total composition. Sesquiterpenes, notably !-caryophyllene and "-caryophyllene, predominate, followed by linalyl acetate, !-pinene, and D-limonene. A nanoemulsion was developed from this essential oil using an environmentally friendly, high-energy method based on ultrasonication. Characterization of the nanoemulsion by dynamic light scattering (DLS) showed that it has nanometer-sized droplets with an average diameter of 149.4 nm and a negative surface charge of -16.8 mV, thus ensuring slightly moderate colloidal stability. In vivo anti-inflammatory activity was evaluated using the carrageenan-induced edema model in NMRI mice. Experimental results showed that after 5 hours, the two highest concentrations of the essential oil (50% and 25 %), as well as the highest concentration of the nanoemulsion (25%), exhibited superior anti-inflammatory activity compared to diclofenac, used as the standard treatment. In parallel, the histological study of tissue toxicity confirmed the absence of signs of acute toxicity after 14 days, thus attesting to the good tolerance of the essential oil under the experimental conditions.
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579.01 
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